Effects of Tricine, Glycine and Tris Buffers on Alkaline Phosphatase Activity
نویسندگان
چکیده
The effect of Tris, Glycine and Tricine on alkaline phosphatase activity was tested at a pH of 8.6, 8.85 and 9.1, using pNPP as substrate. Alkaline phosphatase activity was measured by monitoring the increase in NP absorbance at 420nm using a spectrophotometer. Alkaline phosphatase in Tris buffer at pH 9.1 was found to have the highest activity. MichaelisMenten enzyme kinetic analysis revealed that the presence of Tris produced the highest Vmax and Km values for alkaline phosphatase at each pH tested, while the presence of Glycine produced the lowest. Also, increasing the pH was found to increase both the Vmax and Km.
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